The ovalbumin gene. Insertion of ovalbumin gene sequences in chimeric bacterial plasmids.
نویسندگان
چکیده
منابع مشابه
Ovalbumin-Induced Gene Expression in Asthma
Background: Asthma is a chronic infl ammatory disease that is associated with airway hyperresponsiveness, tissue remodeling, and airway obstruction, and that involves coordinate expression of multiple infl ammatory genes in the lungs. Objective: To evaluate the gene expression pattern in a mouse model of asthma and assess the effect of a new drug, R142571, on the gene expression profi le. Metho...
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The entire chicken ovalbumin gene, accompanied by genomic DNA sequences flanking both termini of the gene and three copies of the herpes simplex virus thymidine kinase gene, has been cloned in plasmid pBR322. This recombinant plasmid was linearized and used to transform thymidine kinase-deficient mouse cells. Thymidine kinase-positive transformants were selected by their ability to grow in the ...
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Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.
متن کاملThe ovalbumin gene. Partial purification of the coding strand.
Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. The concentrations of the ovalbumin DNA sequence in var...
متن کاملThe ovalbumin gene. In vitro enzymatic synthesis and characterization.
Using purified single-stranded ovalbumin complementary DNA (cDNAov) as a template for avian myeloblastosis (AM) virus reverse transcriptase, we have enzymatically synthesized a complete double-stranded cDNAov sequence. Our data suggests that many single-stranded cDNAov molecules contain short double-stranded sequences (hairpins) at their 3' termini capable of acting as primers for synthesis of ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71832-2